α smooth muscle actin α sma  (Cell Signaling Technology Inc)

Journal: Scientific Reports

Article Title: 3-carbamoyl proxyl nitroxide attenuates CCl 4 -induced liver fibrosis in mice through antioxidant-inflammatory regulation of TLR4/NF-κB signaling pathway

doi: 10.1038/s41598-026-46137-1

Figure Lengend Snippet: Effect of 3-CP on ROS content and activation of HSCs. ( A - B ) Fluorescence intensity of DCFH-DA in LX2 cells recorded by fluorescence microscope and flow cytometry (scar bar: 100 μm). ( C ) Relative fluorescence intensity of LX2 cells according to flow cytometry. Data are shown as mean ± SD, n = 3. * p < 0.05 vs. control group; # p < 0.05 vs. LPS group. ( D - E ) qRT-PCR analysis of the effects of 3-CP on the levels of α-SMA and type I collagen in LX2 induced by LPS. Data are shown as mean ± SD, n = 3. * p < 0.05 vs. control group; # p < 0.05 vs. LPS group.

Article Snippet: Primary antibodies against NF-κB p65 (Cat #8242S), α-SMA (Cat# 19245 S), collagen I (Cat# 72026 S) and secondary antibody anti-rabbit IgG (CST#7074S) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Activation Assay, Fluorescence, Microscopy, Flow Cytometry, Control, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: 3-carbamoyl proxyl nitroxide attenuates CCl 4 -induced liver fibrosis in mice through antioxidant-inflammatory regulation of TLR4/NF-κB signaling pathway

doi: 10.1038/s41598-026-46137-1

Figure Lengend Snippet: 3-CP alleviated CCl 4 -induced liver fibrosis in mice. ( A ) Representative results and staining intensity scores of immunohistochemical staining forα-smooth muscle actin (α-SMA) and collagen I in the liver (20x, scar bar: 200 μm; 40x, scar bar: 100 μm). ( B ) Representative Western blot images showing protein expression of α-SMA, collagen I, and GAPDH. ( C ) Quantification of α-SMA protein expression. Data are presented as mean ± SD, n = 3. * p < 0.05, vs. control group; # p < 0.05, vs. model group. ( D ) Quantification of collagen I protein expression. Data are presented as mean ± SD, n = 3. * p < 0.05, vs. control group; # p < 0.05, vs. model group. ( E ) Serum index levels of liver function, and ALT and AST levels. ( F ) Levels of inflammatory factors (IL-1β and IL-6) were detected by ELISA. Data are presented as mean ± SD, n = 3. * p < 0.05, vs. control group; # p < 0.05, vs. model group.

Article Snippet: Primary antibodies against NF-κB p65 (Cat #8242S), α-SMA (Cat# 19245 S), collagen I (Cat# 72026 S) and secondary antibody anti-rabbit IgG (CST#7074S) were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Staining, Immunohistochemical staining, Western Blot, Expressing, Control, Enzyme-linked Immunosorbent Assay